p2x7 transcript  (Santa Cruz Biotechnology)

Journal: Purinergic signalling

Article Title: P2X7 receptor: the regulator of glioma tumor development and survival.

doi: 10.1007/s11302-021-09834-2

Figure Lengend Snippet: Fig. 4 Effect of P2X7 inhibi- tion using BBG on glioma C6 tumor development in vivo. a Brilliant Blue G administra- tion led to reduction of glioma tumor mass. Representative images of control and BBG- treated C6 glioma tumors (n = 9 for control group and n = 10 for BBG-treated group). The significance of the differences was determined using Student’s t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. the respective control. b Representative West- ern blot showing the significant decrease of P2X7 expression in tumor homogenates after BBG treatment. Representative image of P2X7 immunodetection in C6 glioma tumors. c P2X7 inhi- bition resulted in diminished activation of matrix metallopro- teinase-2 in glioma tumors. The significance of the differences was determined with Student’s t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. the respective control. d Western blot analysis of known proteins involved in cell adhesion and EMT signal- ing in C6 tumor homogenates (n = 4). e Western blot analysis of known proteins involved in tumor aggressiveness in C6 tumor homogenates (n = 4)

Article Snippet: Three target-specific RNAi (small interfering RNA) complementary to rat P2X7 transcript (sc-108056) and control nonsilencing RNAi were obtained from Santa Cruz Biotechnology, Inc. (USA).

Techniques: In Vivo, Control, Expressing, Immunodetection, Activation Assay, Western Blot

Journal: Purinergic Signalling

Article Title: P2X7 receptor: the regulator of glioma tumor development and survival

doi: 10.1007/s11302-021-09834-2

Figure Lengend Snippet: Activation of P2X7 receptor led to elevated ROS production and mitochondrial membrane depolarization in C6 cells. Quantitative data are presented as signal relative to control. a DCF-DA fluorescence measurement in C6 glioma cells. Cells stimulated with 100 µM BzATP for 1 h were characterized by increased ROS production compared to control in C6 cells ( n = 3). The significance of the differences was determined with one-way ANOVA with Bonferroni post hoc test: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 vs. control group. b Potential-dependent staining of mitochondria in C6 cells using JC-1. 1-h BzATP (100 µM) stimulation led to considerable mitochondrial membrane depolarization ( n = 3). The significance of the differences was determined with one-way ANOVA with Bonferroni post hoc test: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 vs. control group. c Representative images of MitoTracker Red CMXRos accumulation in C6 cells stimulated with 100 µM BzATP for 1 h. d DCF-DA fluorescence measurement in C6 glioma cells with RNA interference of P2X7 expression ( n = 4). Downregulation of P2X7 led to decreased ROS production in C6 cells after 1-h BzATP (100 µM) stimulation. The significance of the differences was determined using paired t -test: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 vs. the respective control. e Potential-dependent staining of mitochondria using JC-1 dye in C6 cells with RNA interference of P2X7 expression after 1 h of 100 μM BzATP pre-treatment ( n = 3). The amount of depolarized mitochondria was higher in control cells when compared to that in cells with P2X7 downregulation. The significance of the differences was determined using paired t -test: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 vs. the respective control

Article Snippet: Three target-specific RNAi (small interfering RNA) complementary to rat P2X7 transcript (sc-108056) and control non-silencing RNAi were obtained from Santa Cruz Biotechnology, Inc. (USA).

Techniques: Activation Assay, Fluorescence, Staining, Expressing